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pAXY0006

Plasmid

#205575

Purpose

Glucos(1-231)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Inserts

Glucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 F84A

Plasmid

#201082

Purpose

Im7 F84A variant inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7 F84A

Use

Tags

Expression

Bacterial

Mutation

Im7 F84A

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Reverse

Plasmid

#197803

Purpose

Level 1 Module 3 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Esp3I

Plasmid

#197836

Purpose

Level 1 module 5 with Esp3I expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Reverse

Plasmid

#197807

Purpose

Level 1 Module 5 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2T-Reverse

Plasmid

#197800

Purpose

Level 1 Module 2T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-7T-Reverse

Plasmid

#197810

Purpose

Level 1 Module 7T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pH6-mNeonC

Plasmid

#178461

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28a

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeonC

Plasmid

#178463

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Use

Tags

6xHis

Expression

Bacterial

Mutation

Free cysteine at the C-terminus of the dimer

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx2

Plasmid

#202752

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2 (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4-Kina

Plasmid

#202739

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_175

Plasmid

#199107

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_172

Plasmid

#199104

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-Integrase13-9

Plasmid

#197728

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Integrase13 (tomato optimized)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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