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Plasmid#207868PurposeMitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmito-mCherryEA
UseTags4xCox8 mitochondrial targetingExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-Nipbl_BSD_FKBPF36V_EYFP
Plasmid#163882PurposeAcute Depletion of NIPBL-EYFPDepositorInsertNipbl (Nipbl Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEX2T VPS4B, residues 1-86
Plasmid#180639PurposeBacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.DepositorInsertVPS4B (VPS4B Human)
UseTagsGSTExpressionBacterialMutationResidues 1-86PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU424
Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA2
Plasmid#19485DepositorInsertheat shock 70kDa protein 2 (HSPA2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
Plasmid#87694PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Cre-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterChicken beta actinAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Nd1
Plasmid#191523PurposeExpression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vectorDepositorInsertv8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP
Plasmid#199358PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
UseTagsLyn kinase sequence (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9-Puro
Plasmid#135965PurposeExpresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)1
Plasmid#126954PurposeBacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ac-AChBP
Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4A3_I
Plasmid#146581PurposeMammalian Expression of HseIF4A3DepositorInsertHseIF4A3 (EIF4A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only