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Plasmid#52089PurposeExpresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTIMP1 (TIMP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema6c-Fc-His
Plasmid#72167PurposeExpresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6c (Sema6c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
UseTagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG.CASP3-Myc
Plasmid#200119PurposeExpresses C-terminal Myc-tagged caspase 3 in mammalian cellsDepositorInsertcaspase 3 (CASP3 Human)
UseTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 Flag Jip1b
Plasmid#52123Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE
Plasmid#123362PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.DepositorInsertFirefly luciferase
UseCRISPR, Lentiviral, and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-SARS-7
Plasmid#166527PurposescFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKII(1-290)
Plasmid#45065PurposeExpression of constit active CaMKIIDepositorInsertCaM Kinase II (Camk2a Rat)
UseTagsExpressionMammalianMutationtruncated at amino acid 290PromoterRSVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F1
Plasmid#55784PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)DepositorInsert3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
TIMP3-bio-His
Plasmid#53418PurposeExpresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTIMP3 (TIMP3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-TdTomato
Plasmid#176275PurposeViral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 JIP3a
Plasmid#53457Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQ and SV40Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(C358S)
Plasmid#42513DepositorInsertPyruvate Kinase M2 Cys358->Ser
UseRetroviralTagsFlagExpressionMutationChanged Cysteine 358 to SerinePromoterCMVAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKIV
Plasmid#45062PurposeExpression of CaMKIVDepositorInsertCam Kinase IV (Camk4 Mouse)
UseTagsExpressionMammalianMutation*PromoterRSVAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta iSH2)
Plasmid#13432DepositorInsertp85 alpha (delta iSH2) (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationDominant negative p85 alpha. The p110 alpha bindi…PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSA3
Plasmid#52263PurposeBacterial expression of human 6His-tagged SUMO3 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only