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Showing: 10621 - 10650 of 148587 results

  4. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162280-plasmid-map-sequence-id-334621

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  5. CMV-B-GECO1

    Plasmid
    #32448
    Purpose
    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    B-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    CMV
    Available since
    Sept. 30, 2011
    Availability
    Academic Institutions and Nonprofits only
    32448-plasmid-map-sequence-id-122739

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  6. pHW581

    Plasmid
    #198818
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198818-plasmid-map-sequence-id-391699

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  8. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  16. GPR55-Tango

    Plasmid
    #66363
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR55 (GPR55 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pEGFP-N1-TrkB

    Plasmid
    #32500
    Depositor
    Rosalind Segal
    Insert
    TRKB (Ntrk2 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    32500-plasmid-map-sequence-id-246445

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  24. N174-MCS (Puro)

    Plasmid
    #81068
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    81068-plasmid-map-sequence-id-155844

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  27. MIG-Luciferase-IRES-GFP

    Plasmid
    #75021
    Purpose
    Encodes the expression of Luciferase and GFP
    Depositor
    Saradhi Mallampati
    Insert
    Luciferase
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    75021-plasmid-map-sequence-id-337469

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  28. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Tags
    Expression
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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Showing: 10621 - 10650 of 148587 results