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pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7(zebrafish) C49S

Plasmid

#108914

Purpose

Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Use

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 49 to Serine

Promoter

SP6

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDM304_YFP-IqgC

Plasmid

#128697

Purpose

expression of YFP-tagged IqgC in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Insert

iqgC

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM326_DPAKa(GBD)-YFP

Plasmid

#128701

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (I325D)

Plasmid

#121412

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

CUP1 promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (F317D)

Plasmid

#121411

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

CUP1 promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (L105D)

Plasmid

#121410

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

CUP1 promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416)

Plasmid

#121408

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(L313D I314K)

Plasmid

#121390

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Use

Tags

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to L…

Promoter

ADH1 promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1385 TDP43 RRM-GFP VLIM-S

Plasmid

#118792

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All VLIM to S in CTD of TDP43 RRM-GFP reporter

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1384 TDP43 RRM-GFP FYW-L

Plasmid

#118793

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All FYW to L in CTD of TDP43 RRM-GFP reporter

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (L105D)

Plasmid

#121405

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

CUP1 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (F317D)

Plasmid

#121406

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

CUP1 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (I325D)

Plasmid

#121407

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Use

Tags

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

CUP1 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgIdua.CMV/CB-EGFP

Plasmid

#121511

Purpose

Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgIdua

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACEBAC1-CoREST-C

Plasmid

#109159

Purpose

Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only