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Plasmid#186167PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby2
Plasmid#176550PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rock1-GBD (840-110 aa) in pEGFPN1
Plasmid#187279PurposeExpress EGFP-Rock1 GTPase-binding domainDepositorInsertRock1 (Rock1 Mouse)
UseTagsEGFPExpressionMammalianMutationGBD (84-110aa)PromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Cyp26a1
Plasmid#185563PurposeAAV construct to ubiquitously express mouse Cyp26a1DepositorInsertCyp26a1 (Cyp26a1 Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_AmDHN132 (pBS0781)
Plasmid#185253PurposeFor the mammalian expression of the Ammopiptanthus protein DHN_AmDHN132. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDHN_AmDHN132
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-mWasabi
Plasmid#176552PurposeExpression of mWasabi for auxotrophic selection in the absence of leucineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-eCFP
Plasmid#176558PurposeExpression of eCFP for auxotrophic selection in the absence of methionineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-TagRFP657
Plasmid#176560PurposeExpression of TagRFP657 for auxotrophic selection in the absence of lysineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-mnx1:ReaChR-citrine
Plasmid#169046PurposeDestination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.DepositorInsertReaChR-Citrine
UseTagsCitrineExpressionMammalianMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3R2
Plasmid#179163PurposeExpression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3r2
Plasmid#179161PurposeExpression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pWZL N-Myc-hPOT1_K90E-IRES_hygro
Plasmid#166407PurposeExpress hPOT1 K90E in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationK90EPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleInsertIDH2 sgRNA #7 (IDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleInsertIDH1 sgRNA #4 (IDH1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-MIOX only
Plasmid#166683PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertGFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 only
Plasmid#166673PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertWild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNG1304
Plasmid#162488PurposeExpresses HelD K239A from Bacillus subtillisDepositorInsertHelD (helD Bacillus subtillis 168)
UseTags6His-V5ExpressionBacterialMutationChanged Lysine 239 to Alanine (K239A)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only