Addgene

We narrowed to 18,164 results for: omp

Showing: 7041 - 7060 of 18164 results

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pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
TRE-DIO-eYFP

Plasmid

#117383

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV1

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Use

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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wt dynamin 1 pEGFP

Plasmid

#34680

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only
Dynamitin-GFP

Plasmid

#51419

Purpose

expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

Dynamitin (DCTN2 Chicken)

Use

Tags

GFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Use

Tags

Expression

Mutation

*

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Tags

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_RUSC1-1/1

Plasmid

#91379

Purpose

Protein expression and purification of human SH3 domain construct RUSC1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RUSC1-1/1 (RUSC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH Abraxas

Plasmid

#27495

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

Abraxas (FAM175A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pTY153 EGFP-T2A-ORF6 SARS-CoV-2

Plasmid

#204975

Purpose

Co-express EGFP and SARS-CoV-2 ORF6

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCA15-Ubc-NLS- scFV-tdTomato

Plasmid

#199446

Purpose

Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

scFV-tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human ubiquitin C (UBC)

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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