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Plasmid#46776Purposeexpression of WT Rab1a in mammalian cellsDepositorInsertRab1a (RAB1A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…TagsExpressionMutationPromoterContain GG cassette and MCS with LacZ for blue/wh…Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_3xFLAG
Plasmid#101654PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS
Plasmid#199348PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-TEVExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
UseTagsIntein-Chitin-binding domainExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgNT
Plasmid#140238PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNADepositorInsertnon-targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (WT)
Plasmid#73963PurposeSTAG2 cDNA (CCDS43990) wild typeDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationwild typePromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-mScarlet
Plasmid#207960PurposeVector for overexpression of the mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- HIV2-ROD vpx
Plasmid#115816PurposeEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance proteinDepositorInsertHIV2ROD Vpx (M15390)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SlugABE-NNG
Plasmid#214356PurposeExpresses SlugABE-NNGDepositorInsertSlugABE-NNG
UseAAVTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP
Plasmid#199360PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB1
Plasmid#19495DepositorInsertDNAJB1 (DNAJB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha CAT
Plasmid#16392DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationCAT: catalytic domain, S65DPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha dNPS
Plasmid#16391DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationN-terminal pseudosubstrate domain deletion: delet…PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:YFP:Tnos (GB0209)
Plasmid#68219PurposeTranscriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoterDepositorInsertYFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only