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Showing: 7001 - 7020 of 14407 results

  1. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172826-plasmid-map-sequence-id-340920

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  4. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid
    #166854
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    F641E, I645E
    Promoter
    pCu
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166854-plasmid-map-sequence-id-329424

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  5. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

    Plasmid
    #166853
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    F641G, I645G
    Promoter
    pCu
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166853-plasmid-map-sequence-id-329422

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  17. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Use
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

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Showing: 7001 - 7020 of 14407 results