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Plasmid#207099PurposepX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.DepositorInsertACACTTCATTAGGTGCTACT
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD2 N-terminal sgRNA
Plasmid#207098PurposepX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.DepositorInsertTTTTTATCAGAAATCATGAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12720
Plasmid#212704PurposegRNA targeting to the gene 4HPPD locusDepositorInsertgRNA targeting to the gene 4HPPD locus
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fg vector
Plasmid#213464PurposeDestination vector for cloning and transformation into TSI locus 1.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDC1 N-terminal sgRNA
Plasmid#207090PurposepX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.DepositorInsertGTATCCTTCCCAGATCATGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-LASV-GPC-HA-c3xFLAG
Plasmid#215691PurposeProduces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tagDepositorInsertLASV GP-HA-FLAG
UseTagsHA-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAD-dG-F3-H2B-mCherry
Plasmid#206007PurposeG-Deleted Rabies vector expressing H2B-mCherryDepositorInsertH2B-mCherry
UseRabies virusTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
UseTagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-IntHA453-SpyTag
Plasmid#198039PurposeExpression plasmid for producing SpyTagged intiminDepositorInserteaeA
UseTagsSpyTagExpressionBacterialMutationdouble HA tag inserted at position 453; lacks nat…PromotertetAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)
Plasmid#211863PurposeNuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationC492APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
IL1_1
Plasmid#207623PurposeLow copy plasmid expressing mTurquoise2 under the TDH3p promoterDepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterScTDH3Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-AcrIC8-NLS
Plasmid#207395PurposeExpresses AcrIC8 with NLS in mammalian cellsDepositorInsertEF1a-AcrIC8-bGH polyA
UseTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-AcrIE2-NLS
Plasmid#207396PurposeExpresses AcrIE2 with NLS in mammalian cellsDepositorInsertEF1a-AcrIE2-bGH polyA
UseTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-AcrIC9-NLS
Plasmid#207397PurposeExpresses AcrIC9 with NLS in mammalian cellsDepositorInsertEF1a-AcrIC9-bGH polyA
UseTagsNLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV02
Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh
Plasmid#208276PurposeExpresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh
Plasmid#208277PurposeExpresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gRHNO1_B
Plasmid#211614PurposesgRNA-B against RhnoDepositorInsertsgRNA RHNO1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB401
Plasmid#209130PurposeMultipartite assembly of the TU for afpA expression under the regulation of afpA promoter and terminator with the pyr4 auxotrophy marker pyr4 from T. reseeiDepositorInsertPafpA:afpA:TafpA::Ppyr4:pyr4:Tpyr4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only