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Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, ā¦Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-3xGFP
Plasmid#191204PurposeFlpO dependent GFP expressionDepositorInsert3X GFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hĪ±1Ga-pDsRed
Plasmid#45811DepositorInserthĪ±1Ga (CACNA1G Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3B-BE3
Plasmid#113411PurposeExpresses hA3B-BE3 in mammalian cellsDepositorInserthA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-ER-5a
Plasmid#56455PurposeLocalization: Endoplasmic Reticulum, Excitation: 488, Emission: 507DepositorInsertER 5a (CALR Human)
UseTagsmEGFPExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
BSD-CryC
Plasmid#209672PurposeExpresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cellsDepositorInsertCRY2 (CRY2 Mustard Weed)
UseLentiviralTagsSplit-TurboID-C and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CibN
Plasmid#209671PurposeExpresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cellsDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsSplit-TurboID-N and pmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE-EGFP_IRES-Blast
Plasmid#213113PurposeExpression of MinE-EGFP in mammalian cellsDepositorInsertMinE-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 wt
Plasmid#16434DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Paxillin
Plasmid#176106PurposeFocal Adhesion markerDepositorInsertPaxillin (PXN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termiā¦ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E7 TCR
Plasmid#122728PurposeExpress HPV16 E7 TCRDepositorInsertHPV16 E7 TCR
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLOX-gfp-iresTK
Plasmid#12243DepositorInsertGFP, HSV1-TK
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dzCas9-Act3.0
Plasmid#158414PurposeCRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only