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Plasmid#197967PurposeMammalian cell expression of wt 3xFLAG-BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseTags3xFLAGExpressionMammalianMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C.NR
Plasmid#185672PurposeExpresses nuclease active eNme2-C.NR in mammalian cellsDepositorInserteNme2-C.NR nuclease
UseCRISPRTagsExpressionMammalianMutationeNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…PromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT009
Plasmid#171635PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-B2M
mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP
Plasmid#11772DepositorInsertWIP (WIPF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-ZO-2
Plasmid#27422DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-eYFP
Plasmid#104055PurposeAn AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV2, and AAV5InsertEYFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-V1-ORF
Plasmid#73635PurposeGatewayâ„¢ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).DepositorTypeEmpty backboneUseTagsVenus fragment 1 (V1)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-6-C, 16-C
Plasmid#124681PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-V2-ORF
Plasmid#73636PurposeGatewayâ„¢ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneUseTagsVenus fragment 2 (V2)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacZ in pLX304
Plasmid#42560DepositorInsertLacZ
UseLentiviralTags2xV5ExpressionMutationPromoterCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP
Plasmid#199359PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDicer
Plasmid#89144PurposeExpression of hDicer in Sf9 CellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e
Plasmid#138492PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (CP1028)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXsIP GFP-VPS33A
Plasmid#67022Purposehuman VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vectorDepositorInsertVacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
[pSK729] pRK5 HA-FLCN(R164A)
Plasmid#136147Purposemammalian expression of FLCNDepositorInsertFLCN (FLCN Human)
UseTagsHAExpressionMammalianMutationR164APromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot8
Plasmid#37367DepositorInsertHsNot8 (CNOT8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only