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pET14b-PITPalpha N90F

Plasmid

#58257

Purpose

bacterial expression of human PITP alpha N90F

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

N90F (abolishes PI binding)

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha K61A

Plasmid

#58258

Purpose

bacterial expression of human PITP alpha K61A

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter cel-mir-235

Plasmid

#46660

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-235

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-124

Plasmid

#46659

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-124

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pEG593

Plasmid

#40119

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

R274E, K318E, Exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pEG628

Plasmid

#40120

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

contains aa1-244, Exon 2 recoded to be RNAi-resis…

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
CFP-GAI(1-151)

Plasmid

#37308

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pDMG8b

Plasmid

#33180

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 sites: aCacTaCa, aCacTaCa

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG10a

Plasmid

#33185

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG10b

Plasmid

#33186

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 site: aCacTaC

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG10c

Plasmid

#33187

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

LTA site: atacGaT

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1b

Plasmid

#33159

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 sites: aCacTaCa, aCacTaCa

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG4b

Plasmid

#33168

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

ptp-1 3'UTR (ptp-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 sites: CacTaCa, aCacTaCa

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I L30&37P

Plasmid

#22166

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Changed Leucine 30 to Proline and changed leucine…

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
3501 pcDNA3 Bid p15 mIII.4

Plasmid

#8781

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p15 mIII.4 (Bid Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Use

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

Plasmid

#115516

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Expression

Mutation

Promoter

UAS

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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