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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pPICZA_CeTRIC-B1_Del

Plasmid

#87344

Purpose

Inducible expression of CeTRIC-B1 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B1

Use

Tags

polyhistidine

Expression

Yeast

Mutation

Δ248-297

Promoter

AOX1

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Use

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom4.2

Plasmid

#87347

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 4.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

4.2 kbp proximal promoter

Availability

Academic Institutions and Nonprofits only

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pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom0.6

Plasmid

#87353

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

0.6 kbp proximal promoter of human MCT2 (nested d…

Availability

Academic Institutions and Nonprofits only

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pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pUC18-H1 RNAi

Plasmid

#87355

Purpose

RNAi expression vector based on H1 promoter and pUC18 vector backbone

Depositor

Saroj Mathupala

Article

Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

Insert

H1 RNA Promoter from U87MG glioma cells

Use

RNAi; Shrna expression vector

Tags

Expression

Mammalian

Mutation

Promoter

H1 RNA

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(WT)-mCherry

Plasmid

#87356

Purpose

PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(YF)-mCherry

Plasmid

#87357

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src isoform 2 (Src Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Y535F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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TLCV2

Plasmid

#87360

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Insert

Cas9-2A-eGFP

Use

CRISPR and Lentiviral; Doxycycline inducible; egf…

Tags

Cas9-T2A-eGFP

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

AraBad

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GGvT

Plasmid

#87362

Purpose

Expresses GGvT, a tandem homodimer green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GGvT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HisB-GRvT

Plasmid

#87363

Purpose

Expresses GRvT, a tandem heterodimer green and red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GRvT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

AraBad

Availability

Academic Institutions and Nonprofits only

Enlarge