-
Plasmid#28205DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsHA tag and tdtomatoExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.GluSnFr.SV40
Plasmid#100889PurposeAAV expression of GluSnFR, a glutamate sensorDepositorInsertGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM040 [Multiome Perturb-seq sgRNA expression vector]
Plasmid#224898PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experimentsDepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SENP1
Plasmid#17357DepositorInsertSENP1 (SENP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-Oct3/4-IP
Plasmid#13460DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsMyc (6x)ExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-mNeon-TurboID
Plasmid#186610PurposeThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboIDDepositorInsertmNeon-V5-TurboID
UseLentiviralTagsmNeonGreenExpressionMammalianMutationFor reference see Branon et al., 2018PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-EGFP
Plasmid#122842PurposeN-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhygro-Cre
Plasmid#34565DepositorInsertCre
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceMarch 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3XFlag-WT-MEFV
Plasmid#167018PurposeGateway entry plasmid with 3xflag-pyrin (Wild-type)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLHKI-pGFPN3
Plasmid#21917DepositorInsertFull length human HKI in pGFP-N3 (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is full length human HKI in pGFP-N3PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest
Plasmid#79770PurposeStaging plasmid for use in DIP transpositionDepositorTypeEmpty backboneUseSynthetic Biology; Staging plasmid for transposit…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH123
Plasmid#66077PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionDepositorInsertphi80 integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRM3-cpHalo∆-mEGFP
Plasmid#194678PurposeCMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertCHRM3_cpHalo∆
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only