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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ gfp-zASC

Plasmid

#98703

Purpose

GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

Depositor

Anna Huttenlocher

Article

Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

Insert

Apoptosis associated speck like protein containing CARD (pycard Zebrafish)

Use

Zebrafish/xenopus expression

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ Dkk1-flag

Plasmid

#16690

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Dkk1 (DKK1 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Use

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pCS2+ macroH2A1.2-GFP-HA

Plasmid

#30515

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Xenopus/zebrafish expression.

Tags

eGFP and triple HA

Expression

Mammalian

Mutation

NO STOP

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pBSc polyA + Shal1

Plasmid

#16196

Depositor

Larry Salkoff

Article

Wei et al Science. 1990 May 4. 248(4955):599-603.

Insert

Drosophila melanogaster Shaker cognate l (Shal Fly)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
p alpha-actinin:GAL4/UAS:TdTomato

Plasmid

#161712

Purpose

Express TdTomato through amplification of GAL4/UAS system

Depositor

Hollis Cline

Article

Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

Insert

TdTomato, GAL4

Use

Xenopus laevis expression

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

actin-GAL4, UAS

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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XE93 mFZ3-CS2+

Plasmid

#16804

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ3-CS2+ (Fzd3 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
XE239 Fz1-ER

Plasmid

#16816

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER (Fzd1 Rat)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
XE42 Rat frizzled-2-CS2+

Plasmid

#16814

Depositor

Randall Moon

Article

Yang-Snyder et al Curr Biol. 1996 Oct 1. 6(10):1302-6.

Insert

rat frizzled 2 (Fzd2 Rat)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE97 mFZ8-CS2+

Plasmid

#16800

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ8-CS2+ (Fzd8 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
p068 pCS mTRPS1 (M)

Plasmid

#11040

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Two Cys residues at positions 917 and 920 in the …

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
Ncerulean-PHdomain

Plasmid

#66784

Purpose

Expression of PIP2 binding protein in sea urchin embryos

Depositor

Amro Hamdoun

Article

Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

Insert

PLCdelta (PLCD1 Human)

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Mutation

only contains PH domain

Promoter

SP6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
NmCitrine-PHdomain

Plasmid

#66783

Purpose

Expression of PIP2 binding protein in sea urchin embryos

Depositor

Amro Hamdoun

Article

Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

Insert

PLCdelta (PLCD1 Human)

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Mutation

only contains PH domain

Promoter

SP6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
XE95 mFZ6-CS2+

Plasmid

#16802

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ6-CS2+ (Fzd6 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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XE94 mFZ4-CS2+

Plasmid

#16803

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ4-CS2+ (Fzd4 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pOX + JShal XR

Plasmid

#16148

Depositor

Larry Salkoff

Article

Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

Insert

Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
LRP6-pCS2

Plasmid

#27242

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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