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Cag.Luc.puro

Plasmid

#74409

Purpose

Constitutive Luciferase expression in mammalian cells along with puromycin selection

Depositor

Georgios Stathopoulos

Article

Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-RPB1-N-term

Plasmid

#195111

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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PL652

Plasmid

#159456

Purpose

Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-2-RPB1-C-term

Plasmid

#195109

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against last exon of RPB1 (C-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

dCas9-CIBN-2A-Puro

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hORAI1sg

Plasmid

#174326

Purpose

gRNA targeting hORAI1 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

Plasmid

#123360

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

Empty LbCpf1 crRNA cloning cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pYL236

Plasmid

#173184

Purpose

CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

Plasmid

#124549

Purpose

Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

Insert

p65 promoter- p65 CDS-Clover

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-WT

Plasmid

#82030

Purpose

Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Patrick Calsou, Sébastien Britton

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Mutations making the cDNA resistant to a siRNA

Promoter

CMV-tet

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pX459-sgRNA3-RPB1-N-term

Plasmid

#195112

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA1-RPB1-N-term

Plasmid

#195110

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

Plasmid

#177144

Purpose

Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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