Addgene

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Hox D4a

Plasmid

#36469

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox D4a (hoxd4a Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Bacterial

Mutation

Q100H, A101T, G125C and R189W

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGolf-ISH

Plasmid

#15853

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type (Gnal Mouse)

Use

Cloning vector

Tags

Expression

Mutation

F199S

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pISH-Adnp

Plasmid

#74340

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

activity-dependent neuroprotective protein (Adnp Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSLI-2xFKBP-GFP(1463000)

Plasmid

#85789

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R

Use

P. falciparum integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEn-C1.1

Plasmid

#61479

Purpose

Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

Depositor

Holger Puchta

Article

Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

Insert

AtU6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCngA2-ISH

Plasmid

#15855

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pISH-Emx2

Plasmid

#74346

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

empty spiracles homeobox 2 (Emx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx3

Plasmid

#74342

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 3 (Dlx3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx6

Plasmid

#74345

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 6 (Dlx6 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Meis2

Plasmid

#74349

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 2 (Meis2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR

Plasmid

#19986

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pISH-Cux1

Plasmid

#74341

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

cut-like homeobox 1 (Cux1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-B

Plasmid

#105457

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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