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Plasmid#31080DepositorInsertMature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterT7Available sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28(+)-NudC
Plasmid#175022PurposePurification of NudC enzymeDepositorInsertnudC
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT317_pET-PhiC31o-pA
Plasmid#52491Purposefor preparation of PhiC31o mRNADepositorInsertPhiC31o integrase
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-Rac1(WT)
Plasmid#22782DepositorInsertRac1 (wt) (RAC1 Human)
UseTagsCyPetExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
UseTagsFLAG, StrepII, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET30B-PTEN
Plasmid#20741DepositorInsertPTEN (PTEN Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorInsertcsgA (csgA )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21b-P35
Plasmid#11808DepositorInsertP35
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-mCherry
Plasmid#112738PurposeExpress 2(KW)2-mCherry in E. coliDepositorInsert2(KW)2-mCherry
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-YPet
Plasmid#102633PurposeIn vitro expression of YPet from the T7 promoterDepositorInsertYPet
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1D1
Plasmid#199768PurposeExpresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1D1
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-mKate
Plasmid#208763PurposeTo express C2-mKate in bacteriaDepositorInsertC2-mKate (Mfge8 Mouse)
UseTagsmKateExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only