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RBM45_Full-length_pGEX

Plasmid

#135127

Purpose

Recombinant expression of RBM45 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM45 (RBM45 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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NOVA1_Full-length_pGEX

Plasmid

#135147

Purpose

Recombinant expression of NOVA1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NOVA1 (NOVA1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTV-mHdac1-tdiRFP670-PEST2

Plasmid

#122351

Purpose

Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Hdac1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-Y206S

Plasmid

#136435

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y206S (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-V242K

Plasmid

#136436

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

V242K (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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UNK_6xZNF_pGEX

Plasmid

#215703

Purpose

Recombinant expression of UNK with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

UNK (UNK Human)

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Msi1_2xRRM_pGEX

Plasmid

#215704

Purpose

Recombinant expression of mouse Msi1 with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

Musashi-1 (Msi1 Mouse)

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Use

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-wt

Plasmid

#201553

Purpose

Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized for E. coli

Promoter

RBS

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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