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Addgene
Showing: 681 - 700 of 822 results

  1. pExp-Bla

    Plasmid
    #112561
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-Bla and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112561-plasmid-map-sequence-id-217226

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  3. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    t7
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

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  4. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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  5. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

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  16. pTRIPZ (M)-HT-Cbx2

    Plasmid
    #82510
    Purpose
    Expresses Halotag-Cbx2 fusion proteins in mammalian cells
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 2 (CBX2 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…
    Availability
    Academic Institutions and Nonprofits only
    82510-plasmid-map-sequence-id-156621

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  17. pTRIPZ (M)-H2A-HT

    Plasmid
    #82517
    Purpose
    Lentivirus vector. Expresses H2A-Halotag fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Histone H2A
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    82517-plasmid-map-sequence-id-170980

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  18. HT7-mTurquoise2-KDEL

    Plasmid
    #188571
    Purpose
    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag7
    Use
    Tags
    KDEL and mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    188571-plasmid-map-sequence-id-369501

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Showing: 681 - 700 of 822 results