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Plasmid#19454DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
UseTagsExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
px330-CD4
Plasmid#136938PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDHDepositorInsertsgRNA targeting CD4 (CD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB6b
Plasmid#19474DepositorInsertDNAJB6b (DNAJB6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOD2047-bwmControl
Plasmid#100006PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB6a
Plasmid#19475DepositorInsertDNAJB6a (DNAJB6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iC++
Plasmid#98165Purposeartificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iC++ gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDP1.2
Plasmid#171092PurposeExpresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)DepositorInsertAAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
UseSynthetic BiologyTagsEmerald, GemininExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJET-49-DExogron-mNeonGreen-R11a
Plasmid#179903PurposeDonor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsminilAA7-mNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD2049-csnControl
Plasmid#100007PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJET-50-DExogron-mCherry-R11b
Plasmid#179904PurposeDonor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKRQ_11_DExCon-mNeonGreen-R25
Plasmid#179896PurposeDonor with homologous arms for Rab25 to knock in DExCon-mNeonGreen moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-22-DExCon-mNeonGreen-R11a
Plasmid#179897PurposeDonor with homologous arms for Rab11a to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-23-DExCon-mCherry-R11b
Plasmid#179898PurposeDonor with homologous arms for Rab11b to knock in DExCon-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-67-DExCon-mNeonGreen-R11b
Plasmid#179899PurposeDonor with homologous arms for Rab11b to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-59-DExCon-mTagBFP2-R25
Plasmid#179900PurposeDonor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD1988-epiDEG
Plasmid#89357PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPdpy-7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPelt-2Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPH2
Plasmid#19535DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
UseTagsHisExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA8
Plasmid#19541DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-iChloC_CA
Plasmid#98171Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseTagsmCherryExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only