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Plasmid#213372PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertQRFP (QRFP Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
peMBD2mRFP (pc2389)
Plasmid#211573PurposeExpresses fl. MBD2 RFP tagged in mammalian cellsDepositorInsertMBD2
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2401_miRFP-FKBP5x
Plasmid#215118PurposeExpresses miRFP-FKBP-FKBP-FKBP-FKBP-FKBP in mammalian cellsDepositorInsertmiRFP-FKBP-FKBP-FKBP-FKBP-FKBP
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2094_CRY2olig-miRFP
Plasmid#215114PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cellsDepositorInsertCRY2olig-miRFP (CRY2 Mustard Weed)
UseTagsmiRFPExpressionMammalianMutationE490GPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pActin-miRFP704nano
Plasmid#206191PurposeHuman beta-actin labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertmiRFP704nano-human beta-actin
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClathrin-miRFP704nano
Plasmid#206193PurposeClathrin labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertmiRFP704nano-clathrin light chain
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP704nano
Plasmid#206194PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertmiRFP704nano-LC-Myosin
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP704ano
Plasmid#206196PurposeHistone 2B labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertH2B-miRFP704nano
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP704nano
Plasmid#206195PurposeLAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertLAMP1-miRFP704nano
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-iRFP682-N1
Plasmid#197184PurposeExpresses the fusion protein of CytERM-iRFP682 in mammalian cellsDepositorInsertCytERM-iRFP682
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-emiRFP703-N1
Plasmid#197185PurposeExpresses the fusion protein of CytERM-emiRFP703 in mammalian cellsDepositorInsertCytERM-emiRFP703
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRset-rogRFP2
Plasmid#207875PurposeBacterial expression of the red fluorescent rogRFP2 redox sensorDepositorInsertrogRFP2
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-rogRFP2
Plasmid#207876PurposeMammalian expression of the red fluorescent rogRFP2 redox sensorDepositorInsertrogRFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE-RFP1
Plasmid#202154PurposeLevel 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertRFP1 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-mRFP1
Plasmid#202145PurposeLevel 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertmRFP1 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB511-TP-tagRFP
Plasmid#203482PurposeFor Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertTagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pnRFP
Plasmid#201678PurposeBacteria protein expression of pnRFP biosensorDepositorInsertpnRFP
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-pnRFP
Plasmid#201679PurposeExpresses pnRFP in mammalian cellsDepositorInsertpnRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-mRFP
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_RFP-60A
Plasmid#200394PurposePlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.DepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only