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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC50A1_STOP

Plasmid

#161394

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC50A1 (SLC50A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_RB1_p.R451H

Plasmid

#81595

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R451H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.L550I

Plasmid

#81596

Purpose

Gateway Donor vector containing RB1 ,part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L550I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.V654L

Plasmid

#81562

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V654L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.F755I

Plasmid

#81546

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F755I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

Plasmid

#203798

Purpose

Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Article

Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

Inserts

PIK3CA (PIK3CA Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

Plasmid

#203797

Purpose

Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Article

Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

Inserts

PIK3CA (PIK3CA Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.Y756C

Plasmid

#82863

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y756C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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RB1-TP53-LRG-GFP

Plasmid

#225876

Purpose

Guides targeting TP53 and RB1

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

RB1 and TP53 (TP53, RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.E398L

Plasmid

#82847

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E398L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.A772S

Plasmid

#82803

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A772S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1158

Plasmid

#29073

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple30 (CD68 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Aug. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1159

Plasmid

#29074

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple31 (CD68 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1501

Plasmid

#29249

Purpose

Expression of the reporter gene was not detected in the brain, eye and embryo using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple30 (CD68 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:D127-E814 (EIF3B Human)

Use

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_EIF3B:186-685

Plasmid

#211205

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:V186-T685 (EIF3B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EIF3B:186-685

Plasmid

#211279

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:V186-T685 (EIF3B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pETM-41-HsPAN3_N

Plasmid

#147012

Purpose

Bacterial Expression of HsPan3

Depositor

Elisa Izaurralde

Insert

HsPan3 (PAN3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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