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Plasmid#135675PurposeDox-inducible expression (TRE promoter) of C124S mutant Pten cDNA, Blasticidin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Incoherent feedforward (IFF) plasmid
Plasmid#169748PurposeStandalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.DepositorInsertmiR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-TCRb-FKBP-Blasticidin
Plasmid#120723PurposeExpresses ER-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertT cell receptor β chain
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-blast
Plasmid#167825PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-EBFP2-MiniWhite
Plasmid#165918PurposeBlasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
Plasmid#127636PurposeEncodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)DepositorInsertmTurquoiuse2-Tat, YPet-PCP
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-BPII
Plasmid#31632DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutationaa 492-622 of claspinPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
PX387 Mycoplasma mobile Cas9
Plasmid#68324PurposeMycoplasma mobile Cas9DepositorInsertMmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-224N
Plasmid#31634DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutation1st 224 amino acids of claspinPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pWZL Blast2
Plasmid#13609DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-BPI
Plasmid#31633DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutationaa 273-492 of claspinPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pGLAST-DEST (JDW 1009)
Plasmid#229822PurposeA single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Blast-PECAM1-Neo
Plasmid#221149PurposeExpression of platelet and endothelial cell adhesion molecule 1 (CD31)DepositorInsertPlatelet And Endothelial Cell Adhesion Molecule 1 (PECAM1 Human)
UseTagsExpressionMammalianMutationPromoterPECAM1Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAHW/Joseph2-Multitagged Plasmid
Plasmid#226725PurposeValidates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.DepositorInsertpAHW/Joseph2
UseDrosophila s2 cell expressionTagsHA, Myc, GFP, Flag, V5, 6xHisExpressionInsectMutationPromoterAct5CAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Blast FAP S624A
Plasmid#207402PurposeExpresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralTagsExpressionMutationchanged Serine 624 to Alanine. It's a protea…PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Blast FAP A657S
Plasmid#207403PurposeExpresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralTagsExpressionMutationchanged Alanine 657 to Serine. It's an endop…PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only