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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Jun 1. ():.

Insert

Use

Tags

VP16 Fusion Protein

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate2

Plasmid

#25947

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Jun 1. ():.

Insert

Use

Tags

GAL4 Fusion Protein

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET28 His10-FcRɣ

Plasmid

#113021

Purpose

for purification of His10-FcRɣ intracellular domain

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

His10-FcRɣ intracellular domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNuc-TrxRFP1

Plasmid

#98998

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and nuclear localization sequence

Use

Tags

His-tag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

Acetamidase operone

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2R2

Plasmid

#15806

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL

Plasmid

#163574

Purpose

express wild type Dcp2 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTOX2

Plasmid

#127447

Purpose

Encodes MqsR toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAd-DsRed

Plasmid

#17515

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

pAd-DsRed

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pE_Cre_lambdaCos

Plasmid

#173785

Purpose

The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mutation

S102Y and S279Y- please see depositor comments

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFGM1

Plasmid

#64949

Purpose

source of GmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-GmR-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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punc-119cR

Plasmid

#21596

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Jun 8. ():.

Insert

unc-119:mCherry (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

mCherry

Expression

Worm

Mutation

N/A

Promoter

Available since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2U

Plasmid

#15810

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2U (UBE2U Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pLV.CMV.GFP.miR124.sponge

Plasmid

#115975

Purpose

Kockdown of miR-124

Depositor

Johan Jakobsson

Article

Akerblom et al J Neurosci. 2012 Jun 27;32(26):8879-89.

Insert

GFP-miR124sponge

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1813_pCR8-gSMN2-DN1

Plasmid

#118646

Purpose

Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS mNFATc3-EE

Plasmid

#17868

Depositor

Jerry Crabtree

Article

Graef et al Cell. 2001 Jun 29. 105(7):863-75.

Insert

NFATc3 (Nfatc3 Mouse)

Use

Tags

EE

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
RHD_exon1_TALEN_R

Plasmid

#62240

Purpose

expression of TALEN targeting human RHD exon1

Depositor

Hyongbum Kim

Article

Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

Insert

human RHD_exon1_TALEN_Right

Use

TALEN

Tags

HA tag, NLS

Expression

Mutation

Promoter

CMV

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
RHD_exon1_TALEN_L

Plasmid

#62239

Purpose

expression of TALEN targeting human RHD exon1

Depositor

Hyongbum Kim

Article

Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

Insert

human RHD_exon1_TALEN_Left

Use

TALEN

Tags

HA tag, NLS

Expression

Mutation

Promoter

CMV

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-PKCbetaII-YFP

Plasmid

#14866

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

YFP-PKCbetaII-YFP

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pMiLRTetR(L)

Plasmid

#102537

Purpose

Plasmid for testing the activity of the Minos transposon (excision and transposition assays)

Depositor

Michalis Averof, Charalambos Savakis

Article

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposon carrying TetR gene

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1 delta OB

Plasmid

#13241

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 delta OB (POT1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CYPHR

Plasmid

#14864

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

cyan/yellow PH domain reporter (Plcd1 )

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pAC1815_pCR8-gSMN2-DN3

Plasmid

#118648

Purpose

Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18PV-mini-Tn7T

Plasmid

#64956

Purpose

pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAC1803_pmax-RBM38-dCasRx

Plasmid

#118637

Purpose

Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2L6

Plasmid

#15797

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L6 (UBE2L6 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+mTurquoise2-Golgi

Plasmid

#128425

Purpose

Contains insert allowing in-vivo visualization of Golgi apparatus

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

mTurquoise2-Golgi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p159 pPr-luc

Plasmid

#8392

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin promoter (Pbsn Rat)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
pJA317

Plasmid

#74488

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d1ODC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+CyclinB1-GFP

Plasmid

#128426

Purpose

Contains insert allowing in-vivo visualization of CyclinB1 protein (G2/M)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

CyclinB1-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
eNOS S1179A

Plasmid

#22485

Depositor

William Sessa

Article

Fulton et al Nature. 1999 Jun 10. 399(6736):597-601.

Insert

eNOS S1179A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlac01

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+Lifeact-eGFP

Plasmid

#128428

Purpose

Contains insert allowing in-vivo visualization of F-actin

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

Lifeact-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB4A5 JQ929581

Plasmid

#38168

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only
pAC1808_pmax-RBFOX1N-FRB-C

Plasmid

#118641

Purpose

Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-FRB-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM9121_pMOX

Plasmid

#160922

Purpose

Methanol oxidase (MOX) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Methanol oxidase promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLIParaF.Ec-250n

Plasmid

#18855

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

arabinose FRET sensor

Use

Tags

CFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pHR Src-TagBFP

Plasmid

#171036

Purpose

TagBFP-tagged Src kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCSh5-2300

Plasmid

#171788

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1807_pmax-dCasRx-FKBP

Plasmid

#118640

Purpose

Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-FKBP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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