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  17. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

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  34. pYZ675

    Plasmid
    #132954
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132954-plasmid-map-sequence-id-307989

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  35. pYZ694

    Plasmid
    #132956
    Purpose
    crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132956-plasmid-map-sequence-id-307975

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Showing: 1361 - 1400 of 23151 results