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Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorInsertCaspase1 (CASP1 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p38KTRmCerulean3
Plasmid#59149PurposeORF encoding p38 KTR mCerulean3 flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Grx1-roCherry
Plasmid#141068PurposeFluorescent biosensor for 2GSH/GSSG ratioDepositorInsertGrx1-roCherry
UseTagsExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threon…PromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1 Grx1-roCherry-mito
Plasmid#141069PurposeFluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localizationDepositorInsertGrx1-roCherry
UseTagsdMTSExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threoni…PromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Flex-mRuby3-2A
Plasmid#203846PurposeNeuron specific expression of a Cre-dependent mRuby3DepositorInsertmRuby3
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-EGFP
Plasmid#153163PurposeMouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Padron2
Plasmid#172355PurposeExpresses the reversibly switchable fluorescent protein Padron2 in bacterial cellsDepositorInsertPadron2
UseTags6xHisTag (N terminal on backbone)ExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn hPGK1-mRuby
Plasmid#220911PurposeNeuronal specific expression of the fusion construct PGK1-mRubyDepositorInsertPGK1-mRuby (PGK1 Human)
UseAAVTagsmRubyExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA E545K
Plasmid#12525DepositorInsertPIK3CA E545K (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationE545K. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtAcKRS (human-opti)
Plasmid#164195PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansionDepositorInsertMtAcKRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationD76G; L325M; L329I; Y330F; L333A; C372FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-eGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-jGCaMP7f-WPRE
Plasmid#203848PurposeExpression of GCaMP7f in CaMKII expressing cellsDepositorInsertjGCaMP7f
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorInsertHuman PARP1 (PARP1 Human)
UseTagshistidine-tagExpressionBacterialMutationPolymorphism A762PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
Plasmid#103865PurposeCatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).DepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationContains the H133A and H1058A mutations which ina…PromoterEF1aAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only