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Plasmid#76962Purpose3rd generation lentiviral gRNA plasmid targeting human CADDepositorInsertCAD (CAD Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.1
Plasmid#59911PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorInsertsgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP
Plasmid#173029PurposeFor expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.DepositorInsertpspCas13b
UseCRISPR and LentiviralTags3xFLAG, BFP, HIV NES, and T2AExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDK2 gRNA (BRDN0001147786)
Plasmid#77192Purpose3rd generation lentiviral gRNA plasmid targeting human CDK2DepositorInsertCDK2 (CDK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK4 gRNA (BRDN0001146469)
Plasmid#75876Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK4DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MERTK gRNA (BRDN0001146205)
Plasmid#76446Purpose3rd generation lentiviral gRNA plasmid targeting human MERTKDepositorInsertMERTK (MERTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD2 gRNA (BRDN0001146864)
Plasmid#75827Purpose3rd generation lentiviral gRNA plasmid targeting human CD2DepositorInsertCD2 (CD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
BTK gRNA (BRDN0001149473)
Plasmid#77074Purpose3rd generation lentiviral gRNA plasmid targeting human BTKDepositorInsertBTK (BTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK12 gRNA (BRDN0001145847)
Plasmid#75917Purpose3rd generation lentiviral gRNA plasmid targeting human CDK12DepositorInsertCDK12 (CDK12 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATM gRNA (BRDN0001149033)
Plasmid#77531Purpose3rd generation lentiviral gRNA plasmid targeting human ATMDepositorInsertATM (ATM Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLT3 gRNA (BRDN0001145134)
Plasmid#77350Purpose3rd generation lentiviral gRNA plasmid targeting human FLT3DepositorInsertFLT3 (FLT3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgLkb1/Cre
Plasmid#66894PurposeExpresses an Lkb1-targeting gRNA and Cre-recombinaseDepositorInsertsgLkb1
UseCRISPR, Cre/Lox, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATM gRNA (BRDN0001146099)
Plasmid#77530Purpose3rd generation lentiviral gRNA plasmid targeting human ATMDepositorInsertATM (ATM Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK9 gRNA (BRDN0001146478)
Plasmid#77169Purpose3rd generation lentiviral gRNA plasmid targeting human CDK9DepositorInsertCDK9 (CDK9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK3 gRNA (BRDN0001145848)
Plasmid#77166Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK3DepositorInsertEIF2AK3 (EIF2AK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.V5.FLAG_NGFR
Plasmid#158250PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.V5.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
IGF1R gRNA (BRDN0001146028)
Plasmid#76689Purpose3rd generation lentiviral gRNA plasmid targeting human IGF1RDepositorInsertIGF1R (IGF1R Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only