Addgene

We narrowed to 37,276 results for: ina

Showing: 6921 - 6940 of 37276 results

Results per page:

Sort results by:

pCMV-HA-mIFITM3-S38N

Plasmid

#58411

Purpose

Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 38 to Asparagine

Promoter

CMV IE

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,88A

Plasmid

#58404

Purpose

Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K88A

Plasmid

#58402

Purpose

Expresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K104A

Plasmid

#58403

Purpose

Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 104 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pSC-hXRCC1-K176R-IntE2

Plasmid

#52277

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Use

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC-mTDG-K341R-IntE2

Plasmid

#52269

Purpose

Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Use

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

EE

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMCL1 Delta C22

Plasmid

#45820

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

mMCL-1 Delta C22

Use

Tags

Expression

Mammalian

Mutation

C-terminal 22 amino acids are deleted from full l…

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pEG593

Plasmid

#40119

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

R274E, K318E, Exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pEG628

Plasmid

#40120

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)

Use

Gateway destination vector

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

contains aa1-244, Exon 2 recoded to be RNAi-resis…

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
pFL-TK-AID3'UTR-DEL

Plasmid

#19988

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Use

Tags

Expression

Mammalian

Mutation

Deletion

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 Flag-NFRKB delta101

Plasmid

#19790

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

deleted N-terminal 1-101 amino acids

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Tags

Expression

Mutation

Promoter

T7 (inactivated)

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge