Addgene

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Showing: 6921 - 6940 of 11900 results

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p172 Dync1i2.E (Ex 1b)

Plasmid

#26442

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p171 Dync1i1.F

Plasmid

#26441

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform F (Dync1i1 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pEMS2372

Plasmid

#225630

Purpose

High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple389 (ADORA2A Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2371

Plasmid

#225637

Purpose

High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Veillonella parvula SKV38 mdh promoter

Available since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE3G-NS3/4A S139A

Plasmid

#186885

Purpose

Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

HCV NS3/4A protease with S139A mutation

Use

Retroviral

Tags

Expression

Mutation

S139A

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-WHG cGAS-HA

Plasmid

#186867

Purpose

Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

White-handed gibbon cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyADO

Plasmid

#226248

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAO

Plasmid

#226242

Purpose

Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAD

Plasmid

#226241

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-bGH-pA (JDW 1221)

Plasmid

#224527

Purpose

A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

WPRE-bGH

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CB2R-GFP

Plasmid

#208919

Purpose

encodes the human cannabinoid receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

Insert

CNR2 (CNR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

MCV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-TetO8x-CMVmin (JDW 1087)

Plasmid

#224475

Purpose

Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

TetO(8x)-CMV promoter

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

Plasmid

#224491

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Golgi-mTagBFP-P2A-H2A-tdiRFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

Plasmid

#224524

Purpose

A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

rox-3xNLS-mCherry-V5-2xSTOP-rox

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p5E lexOP-c-fos min pro (JDW 1254)

Plasmid

#224477

Purpose

Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexOp

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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