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Plasmid#100033PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.DepositorInsertRhoGC E254D
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE254DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab32 DN
Plasmid#37739DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT33N (dominant negative)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)
Plasmid#79036PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant RhoA 3'UTR
Plasmid#26090DepositorInsertmutant RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationBoth miR-31 binding sites in 3’ UTR mutagenized. …PromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab32 CA
Plasmid#37740DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ79L (constitutive active)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_BFP
Plasmid#45302DepositorInsertBFP
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-KIF13AS1371A tail 361-1749
Plasmid#224411PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsFRB-3mycExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZU-LAMP1-mRuby3
Plasmid#222586PurposeStable expression of LAMP1-mRuby3 in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-LC3
Plasmid#221738PurposeStable expression of mRuby3-LC3 in mammalian cellsDepositorInsertLC3B (Map1lc3b Rat)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Cterm_His
Plasmid#220487Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Nterm_His
Plasmid#220482Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
95.a_pSTC0-zeo
Plasmid#202334PurposeDesign 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert95.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
90.a_pSTC0-zeo
Plasmid#202336PurposeDesign 90.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert90.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
80.a_pSTC0-zeo
Plasmid#202338PurposeDesign 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert80.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
70.a_pSTC0-zeo
Plasmid#202340PurposeDesign 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert70.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
50.a_pSTC0-zeo
Plasmid#202342PurposeDesign 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert50.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
98.a_pSTC0-zeo
Plasmid#202332PurposeDesign 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert98.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only