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pCDNA3-HA-human MYCN-V421D

Plasmid

#74166

Purpose

Mammalian expression of human MycN-VD mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MycN (MYCN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

V421D

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
Nano-lantern-H2B/pcDNA3

Plasmid

#51971

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

human H2B

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pENTR1A-3xFlag-S242R-MEFV

Plasmid

#167020

Purpose

Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Expression

Mutation

serine 242 in arginine (S242R)

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00671

Plasmid

#39722

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6312 (Rfx Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-138-1

Plasmid

#26328

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-138-1 (Mir138-1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-Dicer-delDUF

Plasmid

#51365

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to DUF domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST myc MOV10 K530A

Plasmid

#51719

Purpose

plasmid expresses myc-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

K530A

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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JN402: pMVP (R4-R3) Firefly Luciferase

Plasmid

#121741

Purpose

pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Firefly Luciferase

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-483

Plasmid

#26315

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-483 (MIR483 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 P139A (#625)

Plasmid

#99945

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P139A

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-OSBP

Plasmid

#67677

Purpose

Multisite gateway entry clone for adding C-terminal fusions of OSBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

OSBP

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

aa 87-185 of NP_002547.1

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S137A (#627)

Plasmid

#99943

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S137A

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S498A

Plasmid

#32215

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser498Ala

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S97X)

Plasmid

#73964

Purpose

STAG2 cDNA (CCDS43990) S97X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S97X

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
CMV-Cx35-Halo-C-in

Plasmid

#71511

Purpose

Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Use

Tags

HaloTag (internal to sequence)

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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UKNP4.3.1

Plasmid

#98269

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP3.2.2

Plasmid

#98247

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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LC402: pMVP (L3-L2) eGFP + polyA

Plasmid

#121763

Purpose

pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KG802: pMVP (L3-L2) V5 epitope tag + polyA

Plasmid

#121755

Purpose

pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

V5 epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge