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Plasmid#135489PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCitrineDepositorInserthph SpunH2A/Bpr hph-mCitrine
UseTagsmCitrineExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM30
Plasmid#135490PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mClover3DepositorInserthph SpunH2A/Bpr hph-mClover3
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
UseTagsmCerulean3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-CBD-hGLY
Plasmid#134596PurposeLow Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tagDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
UseTagschitin binding domainExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_004
Plasmid#123961PurposeEncodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertcRosa26 destination
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1-N
Plasmid#128310PurposeExpress hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)
UseTagsHisExpressionBacterialMutationN-terminal domain (OB fold, 1–140 aa)PromoterT7Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2-C
Plasmid#128309PurposeExpress hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP2 C-terminal domain (122–211 aa) (NABP2 Human)
UseTagsHisExpressionBacterialMutationC-terminal domain (122–211 aa)PromoterT7Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GDF11-V5 blast
Plasmid#83098PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorInsertGDF11 (GDF11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5-V5 blast
Plasmid#83100PurposeLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC17
Plasmid#118875Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC16
Plasmid#118870Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
UseTagsEGFP and HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC218
Plasmid#118907Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tagDepositorInsertNcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS10, cleavable with 3C protease, YBBR tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC18
Plasmid#118868Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFPDepositorInsertNcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC171
Plasmid#118888Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tagDepositorInsertNcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; SNAP tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC202
Plasmid#118880Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsmKate2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC139
Plasmid#118872Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3D191stop
Plasmid#110537PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationC-terminal deltion of 191 amino acidsPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSM13
Plasmid#101644PurposeEncodes the fluorescent protein meleCFP for constitutive expression in E. coli.DepositorInsertmeleCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Tam1
Plasmid#101639PurposeEncodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.DepositorInsertTam1
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only