Addgene

Showing: 6881 - 6900 of 11900 results

Results per page:

Sort results by:

pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ERBB4 (E836K)

Plasmid

#29534

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 836 to lysine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pQTEV-EPSTI1

Plasmid

#34826

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

epithelial stromal interaction 1 (EPSTI1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ERBB4 (E452K)

Plasmid

#29529

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 452 to lysine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Use

Tags

Expression

Yeast

Mutation

Promoter

VTC4 (native promoter)

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM358_DPAKa(GBD)-YFP

Plasmid

#128700

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ERBB4 (E542K)

Plasmid

#29530

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 542 to lysine

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh1

Plasmid

#41927

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
mTFP1-Vimentin-7

Plasmid

#55515

Purpose

Localization: Intermediate Filaments, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vimentin (VIM Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E563K)

Plasmid

#29532

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 563 to lysine

Promoter

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-Val15AlafsX11

Plasmid

#24732

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

deleted amino acids 44-62. deletion/frameshift…

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

Plasmid

#110735

Purpose

Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

H2B-mOrange2

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS941 TDP43 RRM-GFP VLIMFYW-S

Plasmid

#121992

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobic residues in IDR1&IDR2 mutated…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1383 TDP43 RRM-GFP FYW-S

Plasmid

#118791

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All FYW to S in CTD of TDP43 RRM-GFP reporter

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1386 TDP43 RRM-GFP VLIM-F

Plasmid

#118794

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All VLIM to F in CTD of TDP43 RRM-GFP reporter

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1387 TDP43 RRM-GFP KRED-S

Plasmid

#118795

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All KRED to S in CTD of TDP43 RRM-GFP reporter

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Cwc25-pRSETA

Plasmid

#61043

Purpose

Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Cwc25

Use

Tags

TEV site, Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only