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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts3

Plasmid

#115864

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Use

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts1

Plasmid

#115862

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts2

Plasmid

#115887

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDESTmycYBX1

Plasmid

#19878

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Y box binding protein 1 (YBX1 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_TFAM_mKate2

Plasmid

#167331

Purpose

Expresses mKate2 fused to full length TFAM in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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MS2-HB

Plasmid

#35573

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

MS2 Coat Protein (cp Bacteriophage)

Use

Retroviral

Tags

HTBH

Expression

Mammalian

Mutation

Insert is a tail-to-head tandem placement of two …

Promoter

CMV

Available since

May 30, 2012

Availability

Academic Institutions and Nonprofits only

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pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Inducible Tac promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

Plasmid

#92099

Purpose

Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

Expression

Mammalian

Mutation

Promoter

Tet-On 3G Bidirectional

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-hTERT

Plasmid

#51637

Purpose

Mammalian expression of 3xHA tagged human TERT, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

TERT (TERT Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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EYFP-JAB1

Plasmid

#111213

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

Insert

CSN5 (COPS5 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3

Plasmid

#191752

Purpose

Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA1A

Plasmid

#19510

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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