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Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pPI528
Plasmid#207442Purpose6xHis-SS-TEV-ErkB-T176A-Y178A bacterial expression vector - catalytically inactiveDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationT176A, Y178APromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-dnHIF
Plasmid#206343PurposeAAV plasmid expressing dominant negative HIF1A in photoreceptorsDepositorInsertdominant negative hif1a
UseAAVTagsExpressionMutationdominant negative HIF1APromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-HAT
Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[K5A]-FLAG
Plasmid#199326Purposemutated ubiquitination site (ubiquitination inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Lysine 5 to AlaninePromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[K5R]-FLAG
Plasmid#199325Purposemutated ubiquitination site (ubiquitination active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Lysine 5 to ArgininePromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcPylRS(Y384F Y306A)
Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193318PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
UseTags4MYCExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193317PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
UseTags4FLAGExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK2A2_IDG-K
Plasmid#189870PurposeGateway destination clone of CSNK2A2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCSNK2A2 (CSNK2A2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-COQ8A-V5-miniTurbo_IDG-K
Plasmid#189885PurposeGateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCOQ8A (COQ8A Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DCLK3_IDG-K
Plasmid#189891PurposeGateway destination clone of DCLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertDCLK3 (DCLK3 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-QPAS1-VP16
Plasmid#186187PurposeAAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activationDepositorInsertNLS-QPAS1-VP16
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Po_P41-Cd4-bio-His
Plasmid#126836PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertP41
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Po_MSP3.5-Cd4-bio-His
Plasmid#126837PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertMSP3.5
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Po_MSP3.6-Cd4-bio-His
Plasmid#126838PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertMSP3.6
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Po_MSP7.12-Cd4-bio-His
Plasmid#126839PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertMSP7.12
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Po_MSP7.8-Cd4-bio-His
Plasmid#126840PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertMSP7.8
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only