Addgene

We narrowed to 18,164 results for: omp

Showing: 6801 - 6820 of 18164 results

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pSB700 Cerulean-Zhang2.0

Plasmid

#79378

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

zhang 2.0

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pHWBF06EC

Plasmid

#130827

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delCC

Plasmid

#130836

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of coiled coil domain

Promoter

CaMV 35S

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delSUNec

Plasmid

#130847

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Deletion of SUN domain

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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SUN2delCCec

Plasmid

#130848

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Expression

Mutation

Deletion of coiled coil domain

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pSD141B

Plasmid

#218139

Purpose

Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56-eGFP-IRES2-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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ABE8e(TadA-8e V106W)

Plasmid

#138495

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e V106W)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

TadA-8e V106W

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Use

Tags

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-B/BRISBANE/60/2008

Plasmid

#187920

Purpose

Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Use

Tags

UNTAGGED

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Use

Tags

UNTAGGED

Expression

Mutation

Promoter

CMV Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Use

Tags

UNTAGGED

Expression

Mutation

Promoter

CMV Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Fusion protein with Cas9

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pRK5

Plasmid

#46333

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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(SIM)5

Plasmid

#126947

Purpose

Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pSLC-255

Plasmid

#124292

Purpose

Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

Insert

vsfGFP-9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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