-
Plasmid#174705PurposeDoxycycline inducible expression of human MeikinDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585
Plasmid#174397PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-BASU-EGFP
Plasmid#163845PurposeIn vitro transcription of BASU-EGFP. Parton lab clone JRRDepositorInsertBASU
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb-EGFP
Plasmid#118752PurposeMultisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEXDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-ccdb
Plasmid#118628PurposeMultisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_S130A
Plasmid#174399PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAPDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationS130APromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_Y167F
Plasmid#174398PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationY167FPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-LifeAct-EGFP
Plasmid#163608PurposeExpresses LifeAct fused to EGFP Flp-dependently in mammalian cellsDepositorInsertLifeAct
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-SQSfl
Plasmid#171010PurposeHiLITR protease with full-length SQS targeting information (ER)DepositorInsertEGFP-uTEV1-SQS(FL)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-TPD54
Plasmid#172454PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsGFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD53
Plasmid#172461PurposeExpression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFPDepositorInsertTPD53 (TPD52L1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD54
Plasmid#172459PurposeExpression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFPDepositorInsertTPD54 (TPD52L2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34W)-PB
Plasmid#171072PurposeA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34WPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34F)-PB
Plasmid#171071PurposeA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34FPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut
Plasmid#169298PurposeConstitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertArid3a-3'UTR-Mut (Arid3a Mouse)
UseLentiviralTagsExpressionMutationMutations in miR-125b-2 and let-7c binding sitesPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut
Plasmid#169296PurposeConstitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertARID3A-3'UTR-Mut (ARID3A Human)
UseLentiviralTagsExpressionMutationMutations in miR-125b-2 and let-7c binding sitesPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2hygro-EGFP-FF2
Plasmid#164921PurposeExpresses EGFP cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only