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Plasmid#213721PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS100
Plasmid#209354PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-moxMaple-GW
Plasmid#209353PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-HALO-GW
Plasmid#209351PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-SNAP-GW
Plasmid#209350PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-His6
Plasmid#212776PurposeC-terminal PCR-based tagging with His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA106
Plasmid#209339PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA105
Plasmid#209338PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA101
Plasmid#209335PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mVenus fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAAA
Plasmid#202587PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28b(+)/ADK C77S
Plasmid#201409PurposeOverexpresses Escherichia coli adenylate kinase C77SDepositorInsertEscherichia coli adenylate kinase C77S (adk Escherichia coli)
UseTagsExpressionBacterialMutationchanged cysteine 77 to serinePromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-TEV219S153N
Plasmid#201488PurposeTANGO detection of V5-tagged proteinDepositorInsertNbV5-TEV219S153N
UseTagsTEV219 S153NExpressionMammalianMutationS153N in TEV219PromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-PMTmApple
Plasmid#203794PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesisDepositorInsertPMT
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mRuby3-WPRE
Plasmid#193336PurposeExpresses mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-YPet-WPRE
Plasmid#193335PurposeExpresses YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mAmetrine1.1-WPRE
Plasmid#193333PurposeExpresses mAmetrine1.1 under the TRE promoter in mammalian cellsDepositorInsertmAmetrine1.1
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mNeonGreen-WPRE
Plasmid#193334PurposeExpresses mNeonGreen under the TRE promoter in mammalian cellsDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only