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Plasmid#158790PurposepcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioIDDepositorInserteIF4G1 (with microexon)
UseTags3xFlag and BirA*ExpressionMammalianMutationPromoterminiCMVAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BA.2.12.1
Plasmid#186809Purposepseudovirus plasmid that expresses spike of BA.2.12.1DepositorInsertSpike (BA.2.12.1) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationL452Q, S704L on the basis of BA.2, c-terminal 18 …PromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-CALR-WT
Plasmid#194578PurposeMammalian Expression of mEGFP-CALR WT Fusion ProteinDepositorInsertCALR (CALR Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-CALR-MUT
Plasmid#194579PurposeMammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion ProteinDepositorInsertCALR (CALR Human)
UseTagsmEGFPExpressionMammalianMutationD387Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T_A1aY1
Plasmid#158751PurposeLentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagRFP-T_A1aY1
UseLentiviralTagsTagRFP-TExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai
Plasmid#202568PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-EGFP-hRhoA-wt
Plasmid#133926PurposeLow level expression of GFP fused human RhoA wild typeDepositorInsertGFP-hRhoA WT (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-tdKatushka2-WPRE
Plasmid#193344PurposeAAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cellsDepositorInserttdKatushka2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-FOS
Plasmid#33353DepositorInsertFOS (Fos Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative of c-Jun; heterodimerizes and i…PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
HDAC3 Flag
Plasmid#13819PurposeMammalian expression of human histone deacetylase 3 with flag tagDepositorInsertHDAC3 (HDAC3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
Beta-Globin-WT
Plasmid#130700PurposeExpresses Beta-Globin WT NMD reporterDepositorInsertBeta Globin (HBB Human)
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shSmad4
Plasmid#37046DepositorInsertSmad4 (SMAD4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbV-EGFP
Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT PINK1 C-myc
Plasmid#13314DepositorInsertPINK1 (PINK1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
HDAC1 Flag
Plasmid#13820PurposeMammalian expression of human histone deacetylase 1 with flag tagDepositorInsertHDAC1 (HDAC1 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2
Plasmid#163403PurposeExpresses BMPR-2 in mammalican cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNox1
Plasmid#58344Purposeexpresses human Nox1 in mammalian cellsDepositorInsertNADPH oxidase 1 (NOX1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM51323
Plasmid#153224PurposeLevel 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminatorDepositorInsertArabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p38KTRmCerulean3
Plasmid#59149PurposeORF encoding p38 KTR mCerulean3 flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only