-
-
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
K15/LacZ
Plasmid#44265DepositorInsertK15 promoter (-4.8) (Krt15 Mouse)
UseMouse Targeting; Ī-galactosidaseTagsLacZExpressionMammalianMutationContains the murine K15 promoter (-4.8) fragmentPromotermurine K15 promoter (-4.8) fragmentAvailable sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1L
Plasmid#33242DepositorInsertATXN1L (ATXN1L Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SF3B1-NTerm
Plasmid#97425PurposeExpresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coliDepositorInsertSF3B1-N-term (AA1-500)
UseTagsGSTExpressionBacterialMutationSilent mutation at nt876, aa289 (AGG to AGA)PromotertacAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SPORT6-RNF114-UIMmut
Plasmid#69923PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationS224PPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL2
Plasmid#164985PurposeNPRL2 expressing vector for mammalian cellsDepositorInsertNPRL2 (NPRL2 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-hCRACR2A-isoform-c
Plasmid#79591PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-c (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p199 Dync1i2.C (Ex1a)
Plasmid#26449DepositorInsertcytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)
UseSubcloning and sequencingTagsExpressionMutationN/APromoterAvailable sinceMarch 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet
Plasmid#130709PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2ĪNSE
Plasmid#74515PurposeExpress GFP-nArgBP2ĪNSE fusion protein in mammalian cells. nArgBP2ĪNSE is the mutant without the neuronal specific exon of nArgBP2.DepositorInsertnArgBP2delNSE (Sorbs2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alaniā¦PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pEGFP-C1-KSENB-Nup133-mut
Plasmid#87339PurposeTo express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changes; deletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE9aP
Plasmid#12434DepositorUseRetroviralTagsHAExpressionMammalianMutationAML1-ETO9a cDNA as in t(8;21) patientsPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h
Plasmid#73066Purposeexpression clone for human SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (SNORD27 Human)
UseTagsno tagExpressionMammalianMutationexpression cassette for SNORD27PromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-GP-2-DNMT3A PWWP
Plasmid#59696PurposeContains histone modification interacting domain (DNMT3A PWWP)DepositorInsertDNMT3A PWWP (Dnmt3a Mouse)
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-2XStrep
Plasmid#125620Purposeexpresses human PPAT protein with a C-terminal 2XStrep affinity tagDepositorInsertPPAT (PPAT Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorInsertCBX7 Chromo (CBX7 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only
