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Plasmid#175508PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLV U6-sgRNA UbC-GFP
Plasmid#106948PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/N-eGFP
Plasmid#183151PurposePlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0.053
Plasmid#119571PurposeLevel 0 part. PromoterDepositorInsertPnirA
UseSynthetic BiologyTagsExpressionMutationRBS* added (22 bp) upstream of ATG.PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-nCas9n-P2A-TagBFP2-pA
Plasmid#176026PurposeMultisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENTDepositorInsertnCas9n-P2A-TagBFP2
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA2
Plasmid#19546DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRai
Plasmid#189938PurposeExpresses CRISPRai cassette under EF1a promoterDepositorInsertCRISPRai cassette
UseTagsExpressionMammalianMutationPromoterCMV enhancer-rat EF1 alphaAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJA3
Plasmid#19520DepositorInsertDnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
UseTagsV5ExpressionMammalianMutationF193L based on NP_005138.3PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAT.015
Plasmid#119558PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only