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Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-pumilioCENP-C
Plasmid#174446Purposein vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_mEGFP-DSep2_Peanut-Strep
Plasmid#174496Purposebacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila PeanutDepositorUseTagsStrep and mEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRabies-EGFP-rtTAn
Plasmid#172119PurposeExpression of EGFP and rtTA-InteinN in Rabies virus infected cellsDepositorInsertEGFP, rtTAn-InteinN
UseRabies virusTagsExpressionMutationPromoterCMVAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
UseTagsExpressionBacterialMutationgfp with first three leucine codons mutated to am…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH805_pJH_LldR_Pcon-sfGFP.0TAG
Plasmid#163736PurposeConstitutive expression of green fluorescent protein encoding zero stop codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH806_pJH_LldR_Pcon-sfGFP.1TAG
Plasmid#163737PurposeConstitutive expression of green fluorescent protein encoding one stop codonDepositorInsertlldr gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH28_pBad_AraC_Para-sfGFP.3TAG
Plasmid#163723PurposeArabinose-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertaraC gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH626_pJH_LldR_PlldR-sfGFP.1TAG
Plasmid#163725PurposeLactate-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertlldr gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH627_pJH_LldR_PlldR-sfGFP.2TAG
Plasmid#163726PurposeLactate-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertlldr gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y135TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH721_pJH_LldR_PlldR-sfGFP.20TAG
Plasmid#163729PurposeLactate-inducible expression of green fluorescent protein encoding twenty amber codonsDepositorInsertlldr gfp.20TAG
UseTagsExpressionBacterialMutationgfp with all 20 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH726_pJH_LldR_PlldR-sfGFP.15TAG
Plasmid#163730PurposeLactate-inducible expression of green fluorescent protein encoding fifteen amber codonsDepositorInsertlldr gfp.15TAG
UseTagsExpressionBacterialMutationgfp with first 15 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH727_pJH_LldR_PlldR-sfGFP.10TAG
Plasmid#163731PurposeLactate-inducible expression of green fluorescent protein encoding ten amber codonsDepositorInsertlldr gfp.10TAG
UseTagsExpressionBacterialMutationgfp with first 10 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH774_pJH_LldR_PlldR-sfGFP.1opal
Plasmid#163733PurposeLactate-inducible expression of green fluorescent protein encoding one opal codonDepositorInsertlldr gfp.1TGA
UseTagsExpressionBacterialMutationgfp with first leucine codon mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
UseTagsExpressionBacterialMutationgfp with first two leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-C-Meikin
Plasmid#174706PurposeDoxycycline inducible expression of human C-Meikin Separase cleavage fragmentDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutation155-373PromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only