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pDisplay-PGD2-1.1-IRES-mcherry

Plasmid

#214763

Purpose

Express GRAB-PGD2-1.1 sensor and membrane tethered mCherry under CMV promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.1 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

Plasmid

#207031

Purpose

Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

Plasmid

#207025

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

W371A I375A D384A Y387A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Pho8-406

Plasmid

#207006

Purpose

Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Osh1-406

Plasmid

#207007

Purpose

Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

OSH1

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-PGD2-1.0-IRES-mcherry

Plasmid

#214762

Purpose

Express GRAB-PGD2-1.0 sensor and membrane tethered mCherry under CMV promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.0 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

Plasmid

#207050

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

P85L P86L

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

Plasmid

#207051

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

T56A R65A T87A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

Plasmid

#207052

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85A P86A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

Plasmid

#207053

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85L P86L

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a-short; U6

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pnCas9-miniSdd7-PBE

Plasmid

#204859

Purpose

For cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd6-PBE

Plasmid

#204858

Purpose

For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78606

Purpose

pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

173CMV, U6

Available since

Dec. 16, 2017

Availability

Academic Institutions and Nonprofits only
p2T-CMV-miniSdd7-BE4max-BlastR

Plasmid

#204851

Purpose

For cytosine base editing using miniSdd7 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-miniSdd6-BE4max-BlastR

Plasmid

#204850

Purpose

For cytosine base editing using miniSdd6 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-R-JAK2-Ddd9-C

Plasmid

#204854

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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