Showing: 661 - 680 of 682 results
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Plasmid#205518PurposeEndosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertendo-Nb80
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
UseTagsNuclear localization signal (NLS) and mCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-P2A-APEX2-VHH[GFP]
Plasmid#126749PurposeExpression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJDepositorInsertVHH[EGFP]
UseZebrafish muscle expression cloneTagsmKate2-P2A-APEX2ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB-_attB53
Plasmid#183615PurposeRMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EGFP–COSMO–PB
Plasmid#169684PurposeCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104WPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34F)-PB
Plasmid#171071PurposeA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34W)-PB
Plasmid#171072PurposeA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34WPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)
Plasmid#160739PurposeExpresses a truncated OGT (4.5 TPRs) fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailabilityAcademic Institutions and Nonprofits only -
(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)
Plasmid#160740PurposeExpresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobodyDepositorInsertHA-nEPEA-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailabilityAcademic Institutions and Nonprofits only -
AA156
Plasmid#216022PurposeFragmid fragment: (Cas protein) Nanobody binds ALFA tagDepositorInsertNbALFA_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)
Plasmid#160741PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#172196PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sitesDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)AvailabilityAcademic Institutions and Nonprofits only -
pAW240
Plasmid#170791PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolutionDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
UseTags6xHis, E-tag, and myc-tagExpressionBacterialMutationPromoterLacI-PlacAvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-TRIM21
Plasmid#207838PurposeInducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsTRIM21
mCherry
hIgG1-FC
VHH-GFP4
UseTagsweak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 661 - 680 of 682 results