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pVH321-1-Tier1-PhCMV-dCas9-3xNLS

Plasmid

#169595

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2386-Tier1-PhCMV-GCaMP6s

Plasmid

#169519

Purpose

Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2385-Tier1-PhCMV-fastFT

Plasmid

#169518

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, fast maturation time

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2384-Tier1-PhCMV-medFT

Plasmid

#169517

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, medium maturation time

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2408-Tier1-PhCMV-TEVsite

Plasmid

#169503

Purpose

Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease cleavage site

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2405-Tier1-PhCMV-TEV

Plasmid

#169500

Purpose

Tier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

Plasmid

#169499

Purpose

Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet-P2aA-PuroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

PRBSA

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

Plasmid

#169498

Purpose

Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven YPet-P2aA-PuroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1023-Tier1-PhCMV-p2A-PuroR

Plasmid

#169497

Purpose

Tier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-PuroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH9-Tier1-PhCMV-3xNLS

Plasmid

#169478

Purpose

Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Insulated ugpBp from E. coli

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC031

Plasmid

#171152

Purpose

pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3-BBa_J23117-mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J3-BBa_J23117

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC034

Plasmid

#171153

Purpose

pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3(106)- BBa_J23111-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J3(106)-BBa_J23111

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC018.AAV

Plasmid

#171146

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC016.109

Plasmid

#171144

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPPC019.AAV

Plasmid

#171147

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge