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Plasmid#142999PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGON4L (GON4L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b sgRNA-2 PX459 plasmid
Plasmid#192250Purposeexpressing sgRNA-2 targeting eIF3b locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b sgRNA-1 PX459 plasmid
Plasmid#192249Purposeexpressing sgRNA-1 targeting eIF3b locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-K705E
Plasmid#116571PurposeLentiviral expression of PIK3CD K705EDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationK705EPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-P864L
Plasmid#116572PurposeLentiviral expression of PIK3CD P864LDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationP864LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-R785W
Plasmid#116573PurposeLentiviral expression of PIK3CD R785WDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationR785WPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-R927H
Plasmid#116574PurposeLentiviral expression of PIK3CD R927HDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationR927HPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-A396T
Plasmid#116562PurposeLentiviral expression of PIK3CD A396TDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationA396TPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-A835T
Plasmid#116563PurposeLentiviral expression of PIK3CD A835TDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationA835TPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD-E873K
Plasmid#116567PurposeLentiviral expression of PIK3CD E873KDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationE873KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RB1_WT
Plasmid#82275PurposeGateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.DepositorInsertRB1 (RB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RB1_WT
Plasmid#81857PurposeGateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.DepositorInsertRB1 (RB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-PIK3CA H1047R
Plasmid#180031PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsHA tagExpressionMammalianMutationH1047RPromoterEF1aAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RB1_p.I782M
Plasmid#81547PurposeGateway Donor vector containing RB1 ,part of the Target Accelerator Plasmid Collection.DepositorInsertRB1 (RB1 Human)
UseGateway entry vectorTagsExpressionMutationI782MPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_RB1#2
Plasmid#174151PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorInsertRB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgRB1#1
Plasmid#174144PurposeLentiviral vector expressing a sgRNA against the human RB1 geneDepositorInsertRB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGDF11 #1
Plasmid#83083PurposeLentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rb379-928/C706F-pGex2TK
Plasmid#21750DepositorInsertRb (RB1 Human)
UseTagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928 of wt Rb …PromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin
Plasmid#223962PurposeDual fluorescent reporter for histone H2B and for exogenous RbDepositorUseTagsYFP and iRFP670ExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192237Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only