-
Plasmid#167827PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-MΔN
Plasmid#31624DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutationaa 899-1206 onlyPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-MiniWhite
Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pecALPS gag-gfp Blasti
Plasmid#115805PurposeExpress gag-gfp fusion driven by EIF1a promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterEIF1aAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-GPAT4
Plasmid#173168PurposeRetroviral vector to express sgRNA resistant HA tagged human GPAT4DepositorInsertGlycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
UseRetroviralTagsHAExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoAmax-BlastR
Plasmid#152996PurposeC-to-T base editorDepositorInsertevoA-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-blast
Plasmid#167820Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptcALPS gag-gfp Blasti
Plasmid#115806PurposeExpress gag-gfp fusion driven by TK promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterTKAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650
Plasmid#24409DepositorInsertCLASP2 (512-650) (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant with only MT plus end trac…PromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV CDHblast OST-Myorg-V5
Plasmid#175171PurposeLentiviral expression of mouse Myorg-V5DepositorInsertMyorg (Myorg Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pWZL Blast2
Plasmid#13594DepositorInsertintegrin beta 6 (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172A
Plasmid#74380PurposeLentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Ala, Ser 172 Ala (Numbering based on MFF …PromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-1–729_Flag2-Blast
Plasmid#175040PurposeMammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-∆ARD_Flag2-Blast
Plasmid#175041PurposeMammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein with deletion of the a…PromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172E
Plasmid#74443PurposeLentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Glu, Ser 172 Glu (Numbering based on MFF …PromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-mCherry-MiniWhite
Plasmid#165911PurposeBlasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas6-KRAB-2a-BlastR
Plasmid#126492PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoterDepositorInsertLmoCas6-KRAB-2a-BlastR
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianMutationPromoterhUbCAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only