Addgene

We narrowed to 751 results for: Anti-CRISPR

Showing: 661 - 680 of 751 results

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pLenti_EF1a_Flag_TP53_P278A

Plasmid

#183113

Purpose

Expresses flag-tagged p53 with P278A mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine, see depositor com…

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-BsaI_pJMP2748

Plasmid

#208888

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Pir dependent origin of replication

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLY093-EFS-BCMA-Blast-WPRE

Plasmid

#192194

Purpose

Lenti-BCMA-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-641 JE-TRAC-BCMA(BB)28z-P2A

Plasmid

#186127

Purpose

BCMA-CAR DNA production

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BCMA-CAR (TNFRSF17 Human)

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLY098-RetroEFS-BCMABBz-WPRE

Plasmid

#192199

Purpose

Retro-BCMACAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Plasmid

#107311

Purpose

Encoding scFv-p65-HSF1 driven by EF1α promoter

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

Plasmid

#192200

Purpose

Retro-BCMACAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-747_CD19-28z_CAR_TFAP4

Plasmid

#207489

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFPTET1CD

Plasmid

#82561

Purpose

Expression vector for antibody-sfGFP-TET1CD.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

scFvGCN4sfGFPTET1CD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pACBE-FSR (pXK-1648)

Plasmid

#208821

Purpose

Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-749_HA-GD2-28z_CAR_TFAP4

Plasmid

#207490

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-852_HA-GD2-28z_TFAP4-HA

Plasmid

#207502

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY094-EFS-CD22-Blast-WPRE

Plasmid

#192195

Purpose

Lenti-CD22-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-CD22-OE-Blast (CD22 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

Plasmid

#192193

Purpose

Lenti-BCMA-CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLY096-RetroEFS-CD22BBz-WPRE

Plasmid

#192197

Purpose

Retro-CD22CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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