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pAAV-syn-AICD-IRES-hrGFP

Plasmid

#107548

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

human synapsin

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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MIS18A_pLENTI-CAG-IRES-GFP

Plasmid

#176996

Purpose

Mammalian lentiviral expression vector encoding MIS18A

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MIS18A (MIS18A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

Plasmid

#185717

Purpose

AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ala 11 to Pro, Val 70 to Pro

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

Plasmid

#155010

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-dsRed-UBC-GFP-W

Plasmid

#24526

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Type

Empty backbone

Use

Lentiviral

Tags

DsRed and GFP

Expression

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)EGFP/CMV/WPRE∆U3

Plasmid

#41790

Purpose

Lentiviral vector for CMV promoter driven expression of EGFP

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RUNX1-MUT

Plasmid

#194575

Purpose

Mammalian Expression of mEGFP-RUNX1 Mutant (G363Afs*229) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

G363Afs*229

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pUC57mini ZM249M P90A _env eGFP

Plasmid

#132939

Purpose

pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

HIV-1ZM249M provirus (P90A) with env deletion, GFP

Use

Lentiviral

Tags

Expression

Mutation

P90A

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

Plasmid

#209081

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

A53T, NAC deletion (aa60-95)

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.EF1a.eGFP.WPRE.rBG

Plasmid

#105547

Purpose

AAV expression of EGFP from EF1a promoter

Depositor

James M. Wilson

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Flag-ER-Hoxb8-IRES-GFP

Plasmid

#222292

Purpose

Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

David Sykes, Mark Kamps

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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P_EGFP_AC

Plasmid

#185973

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PR-Egfp

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PR

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PALI1_pHIVEGFP

Plasmid

#191252

Purpose

Expresses PALI1 in mammalian cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

NA

Promoter

EF1alpha

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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